Journal of Lipid Research, Vol 21, 1128-1131, Copyright © 1980 by Lipid Research, Inc.
Estimation of phosphatidylglycerol in fluids containing pulmonary surfactant
G Cernansky, DF Liau, SA Hashim and SF Ryan
A simple, direct and non-destructive method for quantitative separation of
phosphatidylglycerol from other phospholipids in pulmonary washings is
described. Total lipid extracts from dog lung washings and
phosphatidylglycerol standard were spotted quantitatively on chromatoplates
and separated by one-dimensional thin-layer chromatography in
chloroform-methanol-water 65:25:4 (v/v/v). Quantification was performed
with Rhodamine 6G and fluorometry. Washings from eleven dogs contained
(mean +/- S.E.) 236 +/- 25 microgram phosphatidylglycerol per g of
parenchymal wet lung tissue which accounted for 8.7% +/- 2.3 of the total
phospholipids. The procedure is especially useful for quantification of
phosphatidylglycerol in microgram amounts.
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