Journal of Lipid Research, Vol 21, 1132-1136, Copyright © 1980 by Lipid Research, Inc.
A technique to study the relationship between adipose cell size and lipogenesis in a heterogeneous population of adipose cells
RL Hood and RF Thornton
A technique is described for the calculation of the incorporation of
radioactive substrate into lipid in adipose cells that have been isolated
and separated into groups according to their diameter from a single sample
of adipose tissue containing a heterogeneous population of cells. After
incorporation of radioactive substrate, a section of adipose tissue is
fixed in osmium tetroxide and the fixed cells isolated and separated into
specific diameter ranges using a series of nylon screens. The separated
cells are weighed, decolorized with hydrogen peroxide, and the
radioactivity is determined in the cells from each diameter range. With
this method, true comparisons can be made between adipose cell size and
lipogenic activity of isolated cells of known diameter which have been
subjected to various nutritional or hormonal treatments. Results with sheep
adipose tissue show that large adipose cells are considerably more active
in the synthesis of lipid than small cells from the same sample of adipose
tissue.
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