Journal of Lipid Research, Vol 22, 131-137, Copyright © 1981 by Lipid Research, Inc.
Free radical label: new approach to the study of super-slow molecular dynamics of lipid systems
VI Gol'danskii, AI Mikhailov, VG Omel'yanenko, VN Smirnov and VP Torchilin
A new method of EPR-spectroscopy, the recombination of free radicals
appearing as a result of indirect radiolysis of biological molecules after
a low temperature irradiation, was applied to the study of molecular
dynamics of dimyristoyl phosphatidylcholine in mass and in the structure of
liposomes above and below the transition temperature. It was shown that the
mobility of lipid molecules in crystalline liposomes was lower than in the
structure of liquid-crystalline liposomes. The addition of cholesterol in
liposome membranes decreased the lateral molecular motion of lipids in
crystalline and liquid- crystalline states; in the latter case, the effect
of cholesterol addition was more pronounced. The activation energy for the
displacement of the fragments of lipid molecules and the lipid molecule as
a whole was estimated, and it was shown that the lipid matrix possesses a
high degree of heterogeneity. The solubility of oxygen in the lipid bilayer
and the mechanism of lipid diffusion are discussed.
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