| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 22, 339-358, Copyright © 1981 by Lipid Research, Inc.
MJ Chapman, S Goldstein, D Lagrange and PM Laplaud
A density gradient ultracentrifugal procedure is described for the rapid
and reproducible isolation of the major lipoprotein classes, VLDL, LDL,
HDL2, and HDL3, from human serum. A step gradient is constructed from four
NaCl/KBr solutions varying in density from 1.006 to 1.24 g/ml and from 3 ml
of serum adjusted to d 1.21 g/ml. Separation is achieved after a single
ultracentrifugation for some 56 x 10(7) gavg min at 15 degrees C in a
swinging bucket rotor, at which time the lipoproteins band isopycnically
and albumin and other serum proteins are sedimented. Densitometric scanning
of gradients revealed a lipoprotein mass profile distinguished by four
absorption maxima which fell within the hydrated density ranges of VLDL (d
less than 1.016 g/ml), LDL (1.028-1.050 g/ml), HDL2 (1.066-1.100 g/ml), and
HDL3 (1.100- 1.153 g/ml). Fractionation of gradients on the basis of band
distribution, followed by chemical, physical, and immunological analyses of
the four principal fractions (i.e., bands) provided data on their
electrophoretic mobility, chemical composition, morphology and size
distribution, immunological reactivity and apolipoprotein content, thereby
confirming their identities as VLDL, LDL, HDL2, and HDL3. The validity of
this separation was supported by the quantitative distribution of apo B and
apo A-I as assessed by radial immunodiffusion. Lipoprotein quantitation
based on chemical analysis of gradient fractions was compared with that by
analytical ultracentrifugation for a group of normolipidemic males; results
concorded well, giving a similar HDL2:HDL3 ratio (0.35-0.36). Our procedure
thus provides a simple and precise manner in which to assess the
lipoprotein and apolipoprotein profile of human serum quantitatively and
qualitatively.
ARTICLES
A density gradient ultracentrifugal procedure for the isolation of the major lipoprotein classes from human serum
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. D. Wool, C. A. Reardon, and G. S. Getz Apolipoprotein A-I mimetic peptide helix number and helix linker influence potentially anti-atherogenic properties J. Lipid Res., June 1, 2008; 49(6): 1268 - 1283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hirano, K. Nohtomi, S. Koba, A. Muroi, and Y. Ito A simple and precise method for measuring HDL-cholesterol subfractions by a single precipitation followed by homogenous HDL-cholesterol assay J. Lipid Res., May 1, 2008; 49(5): 1130 - 1136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Van Linthout, F. Spillmann, A. Riad, C. Trimpert, J. Lievens, M. Meloni, F. Escher, E. Filenberg, O. Demir, J. Li, et al. Human Apolipoprotein A-I Gene Transfer Reduces the Development of Experimental Diabetic Cardiomyopathy Circulation, March 25, 2008; 117(12): 1563 - 1573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Catalano, E. Duchene, Z. Julia, W. Le Goff, E. Bruckert, M. J. Chapman, and M. Guerin Cellular SR-BI and ABCA1-mediated cholesterol efflux are gender-specific in healthy subjects J. Lipid Res., March 1, 2008; 49(3): 635 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kontush, P. Therond, A. Zerrad, M. Couturier, A. Negre-Salvayre, J. A. de Souza, S. Chantepie, and M. J. Chapman Preferential Sphingosine-1-Phosphate Enrichment and Sphingomyelin Depletion Are Key Features of Small Dense HDL3 Particles: Relevance to Antiapoptotic and Antioxidative Activities Arterioscler. Thromb. Vasc. Biol., August 1, 2007; 27(8): 1843 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Delvecchio, P. Bilan, K. Radford, J. Stephen, B. L. Trigatti, G. Cox, K. Parameswaran, and J. P. Capone Liver X Receptor Stimulates Cholesterol Efflux and Inhibits Expression of Proinflammatory Mediators in Human Airway Smooth Muscle Cells Mol. Endocrinol., June 1, 2007; 21(6): 1324 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, A. M. Ahmed, N. McFarlane, C. Capone, D. R. Boreham, R. Truant, S. A. Igdoura, and B. L. Trigatti Regulation of SR-BI-mediated selective lipid uptake in Chinese hamster ovary-derived cells by protein kinase signaling pathways J. Lipid Res., February 1, 2007; 48(2): 405 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. C. McPherson, I. S. Young, B. McKibben, and J. McEneny High density lipoprotein subfractions: isolation, composition, and their duplicitous role in oxidation J. Lipid Res., January 1, 2007; 48(1): 86 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kontush and M. J. Chapman Functionally Defective High-Density Lipoprotein: A New Therapeutic Target at the Crossroads of Dyslipidemia, Inflammation, and Atherosclerosis Pharmacol. Rev., September 1, 2006; 58(3): 342 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bach-Ngohou, K. Ouguerram, R. Frenais, P. Maugere, B. Ripolles-Piquer, Y. Zair, M. Krempf, and J. M. Bard Influence of Atorvastatin on Apolipoprotein E and AI Kinetics in Patients with Type 2 Diabetes J. Pharmacol. Exp. Ther., October 1, 2005; 315(1): 363 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-M. Tandia, C. Lonez, M. Vandenbranden, J.-M. Ruysschaert, and A. Elouahabi Lipid Mixing between Lipoplexes and Plasma Lipoproteins Is a Major Barrier for Intravenous Transfection Mediated by Cationic Lipids J. Biol. Chem., April 1, 2005; 280(13): 12255 - 12261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. SUNDL, U. RESCH, A. R. BERGMANN, J. M. ROOB, and B. M. WINKLHOFER-ROOB The Decrease in {gamma}-Tocopherol in Plasma and Lipoprotein Fractions Levels Off within Two Days of Vitamin E Supplementation Ann. N.Y. Acad. Sci., December 1, 2004; 1031(1): 378 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Hansel, P. Giral, E. Nobecourt, S. Chantepie, E. Bruckert, M. J. Chapman, and A. Kontush Metabolic Syndrome Is Associated with Elevated Oxidative Stress and Dysfunctional Dense High-Density Lipoprotein Particles Displaying Impaired Antioxidative Activity J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 4963 - 4971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Geiss, S. Bremer, P. H. R. Barrett, C. Otto, and K. G. Parhofer In vivo metabolism of LDL subfractions in patients with heterozygous FH on statin therapy: rebound analysis of LDL subfractions after LDL apheresis J. Lipid Res., August 1, 2004; 45(8): 1459 - 1467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Witte, M. R. Taskinen, H. Perttunen-Nio, A. van Tol, S. Livingstone, and H. M. Colhoun Study of agreement between LDL size as measured by nuclear magnetic resonance and gradient gel electrophoresis J. Lipid Res., June 1, 2004; 45(6): 1069 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kontush, S. Chantepie, and M. J. Chapman Small, Dense HDL Particles Exert Potent Protection of Atherogenic LDL Against Oxidative Stress Arterioscler. Thromb. Vasc. Biol., October 1, 2003; 23(10): 1881 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Guerin, W. Le Goff, E. Frisdal, S. Schneider, D. Milosavljevic, E. Bruckert, and M. J. Chapman Action of Ciprofibrate in Type IIB Hyperlipoproteinemia: Modulation of the Atherogenic Lipoprotein Phenotype and Stimulation of High-Density Lipoprotein-Mediated Cellular Cholesterol Efflux J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3738 - 3746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Guerin, P. Egger, W. Le Goff, C. Soudant, R. Dupuis, and M. J. Chapman Atorvastatin Reduces Postprandial Accumulation and Cholesteryl Ester Transfer Protein-Mediated Remodeling of Triglyceride-Rich Lipoprotein Subspecies in Type IIB Hyperlipidemia J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 4991 - 5000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chetiveaux, H. Nazih, V. Ferchaud-Roucher, G. Lambert, Y. Zair, M. Masson, K. Ouguerram, D. Bouhours, and M. Krempf The differential apoA-I enrichment of pre{beta}1 and {alpha}HDL is detectable by gel filtration separation J. Lipid Res., November 1, 2002; 43(11): 1986 - 1993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Guerin, P. Egger, C. Soudant, W. Le Goff, A. van Tol, R. Dupuis, and M. J. Chapman Cholesteryl ester flux from HDL to VLDL-1 is preferentially enhanced in type IIB hyperlipidemia in the postprandial state J. Lipid Res., October 1, 2002; 43(10): 1652 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kolleck, H. Wissel, F. Guthmann, M. Schlame, P. Sinha, and B. Rustow HDL-Holoparticle Uptake by Alveolar Type II Cells . Effect of Vitamin E Status Am. J. Respir. Cell Mol. Biol., July 1, 2002; 27(1): 57 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Tsimihodimos, S.-A. P. Karabina, A. P. Tambaki, E. Bairaktari, G. Miltiadous, J. A. Goudevenos, M. A. Cariolou, M. J. Chapman, A. D. Tselepis, and M. Elisaf Altered distribution of platelet-activating factor- acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia J. Lipid Res., February 1, 2002; 43(2): 256 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sawle, M. K. Higgins, M. P. Olivant, and J. A. Higgins A rapid single-step centrifugation method for determination of HDL, LDL, and VLDL cholesterol, and TG, and identification of predominant LDL subclass J. Lipid Res., February 1, 2002; 43(2): 335 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. PERLEMUTER, A. SABILE, P. LETTERON, G. VONA, A. TOPILCO, Y. CHRETIEN, K. KOIKE, D. PESSAYRE, J. CHAPMAN, G. BARBA, et al. Hepatitis C virus core protein inhibits microsomal triglyceride transfer protein activity and very low density lipoprotein secretion: a model of viral-related steatosis FASEB J, February 1, 2002; 16(2): 185 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Chisholm, E. R. Burleson, G. S. Shelness, and J. S. Parks ApoA-I secretion from HepG2 cells: evidence for the secretion of both lipid-poor apoA-I and intracellularly assembled nascent HDL J. Lipid Res., January 1, 2002; 43(1): 36 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Tselepis, S.-A. P. Karabina, D. Stengel, R. Piedagnel, M. J. Chapman, and E. Ninio N-linked glycosylation of macrophage-derived PAF-AH is a major determinant of enzyme association with plasma HDL J. Lipid Res., October 1, 2001; 42(10): 1645 - 1654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Garner, D. J. Harvey, L. Royle, M. Frischmann, F. Nigon, M. J. Chapman, and P. M. Rudd Characterization of human apolipoprotein B100 oligosaccharides in LDL subfractions derived from normal and hyperlipidemic plasma: deficiency of {alpha}-N-acetylneuraminyllactosyl-ceramide in light and small dense LDL particles Glycobiology, October 1, 2001; 11(10): 791 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Frénais, H. Nazih, K. Ouguerram, C. Maugeais, Y. Zaïr, J. M. Bard, B. Charbonnel, T. Magot, and M. Krempf In Vivo Evidence for the Role of Lipoprotein Lipase Activity in the Regulation of Apolipoprotein AI Metabolism: A Kinetic Study in Control Subjects and Patients with Type II Diabetes Mellitus J. Clin. Endocrinol. Metab., May 1, 2001; 86(5): 1962 - 1967. [Abstract] [Full Text]
|
|
|
|
|
|
M. Guerin, W. Le Goff, T. S. Lassel, A. Van Tol, G. Steiner, and M. J. Chapman Proatherogenic Role of Elevated CE Transfer From HDL to VLDL1 and Dense LDL in Type 2 Diabetes : Impact of the Degree of Triglyceridemia Arterioscler. Thromb. Vasc. Biol., February 1, 2001; 21(2): 282 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Goessling and S. D. Zucker Role of apolipoprotein D in the transport of bilirubin in plasma Am J Physiol Gastrointest Liver Physiol, August 1, 2000; 279(2): G356 - G365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Witt, I. Kolleck, and B. Rüstow Identification of High Density Lipoprotein-Binding Proteins, Including a Glycosyl Phosphatidylinositol-Anchored Membrane Dipeptidase, in Rat Lung and Type II Pneumocytes Am. J. Respir. Cell Mol. Biol., June 1, 2000; 22(6): 739 - 746. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Sambandam, M. A. Abrahani, S. Craig, O. Al-Atar, E. Jeon, and B. Rodrigues Metabolism of VLDL is increased in streptozotocin-induced diabetic rat hearts Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H1874 - H1882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Schamberger, H. C. Geiss, M. M. Ritter, P. Schwandt, and K. G. Parhofer Influence of LDL apheresis on LDL subtypes in patients with coronary heart disease and severe hyperlipoproteinemia J. Lipid Res., May 1, 2000; 41(5): 727 - 733. [Abstract] [Full Text]
|
|
|
|
|
|
M. Guerin, T. S. Lassel, W. Le Goff, M. Farnier, and M. J. Chapman Action of Atorvastatin in Combined Hyperlipidemia : Preferential Reduction of Cholesteryl Ester Transfer From HDL to VLDL1 Particles Arterioscler. Thromb. Vasc. Biol., January 1, 2000; 20(1): 189 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Skoglund-Andersson, R. Tang, M. G. Bond, U. de Faire, A. Hamsten, and F. Karpe LDL Particle Size Distribution Is Associated With Carotid Intima-Media Thickness in Healthy 50-Year-Old Men Arterioscler. Thromb. Vasc. Biol., October 1, 1999; 19(10): 2422 - 2430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Frénais, K. Ouguerram, C. Maugeais, J. S. Marchini, P. Benlian, J. M. Bard, T. Magot, and M. Krempf Apolipoprotein A-I kinetics in heterozygous familial hypercholesterolemia: a stable isotope study J. Lipid Res., August 1, 1999; 40(8): 1506 - 1511. [Abstract] [Full Text]
|
|
|
|
|
|
L. Chancharme, P. Therond, F. Nigon, S. Lepage, M. Couturier, and M. J. Chapman Cholesteryl Ester Hydroperoxide Lability Is a Key Feature of the Oxidative Susceptibility of Small, Dense LDL Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 810 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Teh, P. J. Dolphin, W. C. Breckenridge, and M.-H. Tan Human plasma CETP deficiency: identification of a novel mutation in exon 9 of the CETP gene in a Caucasian subject from North America J. Lipid Res., February 1, 1998; 39(2): 442 - 456. [Abstract] [Full Text]
|
|
|
|
|
|
D. C. Tsoukatos, M. Arborati, T. Liapikos, K. L. Clay, R. C. Murphy, M. J. Chapman, and E. Ninio Copper-Catalyzed Oxidation Mediates PAF Formation in Human LDL Subspecies : Protective Role of PAF:Acetylhydrolase in Dense LDL Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3505 - 3512. [Abstract] [Full Text]
|
|
|
|
|
|
S. K. M. Koller-Lucae, H. Schott, and R. A. Schwendener Interactions with Human Blood in Vitro and Pharmacokinetic Properties in Mice of Liposomal N4-Octadecyl-1-beta -D-arabinofuranosylcytosine, A New Anticancer Drug J. Pharmacol. Exp. Ther., September 1, 1997; 282(3): 1572 - 1580. [Abstract] [Full Text]
|
|
|
|
|
|
S. Goulinet and M. J. Chapman Plasma LDL and HDL Subspecies Are Heterogenous in Particle Content of Tocopherols and Oxygenated and Hydrocarbon Carotenoids: Relevance to Oxidative Resistance and Atherogenesis Arterioscler. Thromb. Vasc. Biol., April 1, 1997; 17(4): 786 - 796. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Barba, F. Harper, T. Harada, M. Kohara, S. Goulinet, Y. Matsuura, G. Eder, Zs. Schaff, M. J. Chapman, T. Miyamura, et al. Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets PNAS, February 18, 1997; 94(4): 1200 - 1205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Guerin, E. Bruckert, P. J. Dolphin, G. Turpin, and M. J. Chapman Fenofibrate Reduces Plasma Cholesteryl Ester Transfer From HDL to VLDL and Normalizes the Atherogenic, Dense LDL Profile in Combined Hyperlipidemia Arterioscler. Thromb. Vasc. Biol., June 1, 1996; 16(6): 763 - 772. [Abstract] [Full Text]
|
|
|
|
|
|
S. Braschi, L. Lagrost, E. Florentin, C. Martin, A. Athias, P. Gambert, M. Krempf, C. Lallemant, and B. Jacotot Increased Cholesteryl Ester Transfer Activity in Plasma From Analbuminemic Patients Arterioscler. Thromb. Vasc. Biol., March 1, 1996; 16(3): 441 - 449. [Abstract] [Full Text]
|
|
|
|
|
|
A. D. Tselepis, C. Dentan, S.-A. P. Karabina, M. J. Chapman, and E. Ninio PAF-Degrading Acetylhydrolase Is Preferentially Associated With Dense LDL and VHDL-1 in Human Plasma : Catalytic Characteristics and Relation to the Monocyte-Derived Enzyme Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1764 - 1773. [Abstract] [Full Text]
|
|
|
|
|
|
M. Guerin, P. J. Dolphin, C. Talussot, J. Gardette, F. Berthezene, and M. J. Chapman Pravastatin Modulates Cholesteryl Ester Transfer From HDL to ApoB-Containing Lipoproteins and Lipoprotein Subspecies Profile in Familial Hypercholesterolemia Arterioscler. Thromb. Vasc. Biol., September 1, 1995; 15(9): 1359 - 1368. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
