J. Lipid Res.
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Journal of Lipid Research, Vol 22, 359-363, Copyright © 1981 by Lipid Research, Inc.

ARTICLES

Evaluation of a high-performance liquid chromatography method for isolation and quantitation of cholesterol and cholesteryl esters

RM Carroll and LL Rudel

A rapid method for the separation and quantitation of unesterified cholesterol and cholesteryl esters by high-performance liquid chromatography is described. All of the cholesteryl esters typically present in plasma were resolved on Zorbax ODS reversed-phase columns using a linear gradient of water (3%-0%) in acetonitrile- tetrahydrofuran 65:35 (v/v) as eluting solvent. Results obtained by this method show good agreement with chemical and gas-liquid chromatographic methods for quantitation of cholesterol and determination of cholesteryl ester percentage compositions. High- performance liquid chromatography provides the only available method for the separation and quantitation of individual cholesteryl esters from lipid extracts of biological samples.
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Copyright © 1981 by the American Society for Biochemistry and Molecular Biology.