Journal of Lipid Research, Vol 22, 377-381, Copyright © 1981 by Lipid Research, Inc.
A sensitive method for the determination of free fatty acids in plasma
WT Hron and LA Menahan
A sensitive micromethod for the colorimetric determination of free fatty
acids (FFA) in plasma has been developed. FFA are extracted with
chloroform-heptane-methanol with silicic acid being added to eliminate
interfering phospholipids. Diphenylcarbazide containing diphenylcarbazone
has been chosen as color-developing reagent, since the chromophore formed
in the presence of FFA-Cu complex is stable and sensitive. Comparison of
the proposed method and a non-routine reference procedure involving, among
other steps, isolation of FFA on thin-layer chromatography would indicate
that the efficiency of extraction of FFA in the proposed method was 90%
that of the reference procedure. The proposed method offers the
sensitivity, convenience, and accuracy needed for the determination of FFA
in situations where the size of sample available is limiting, as with small
rodents.
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