Journal of Lipid Research, Vol 22, 382-386, Copyright © 1981 by Lipid Research, Inc.
Catabolism of human very low density lipoproteins in vitro: a fluorescent phospholipid method for monitoring lipolysis
MR Taskinen, JD Johnson, ML Kashyap, K Shirai, CJ Glueck and RL Jackson
The catabolism of human plasma very low density lipoproteins (VLDL) by
purified bovine milk lipoprotein lipase has been measured in vitro using a
fluorescent phospholipid as a method to monitor lipolysis. Dansyl
phosphatidylethanolamine (DPE) was incorporated into VLDL to form DPE-VLDL,
and the rate of catabolism was followed by measuring the increase in
fluorescence at 490 nm after the addition of the enzyme. The studies were
performed with VLDL isolated from 20 normal individuals. In addition, the
VLDL from 8 mildly obese subjects with primary hypertriglyceridemia (Type
IV phenotype) was studied. With this in vitro system and with a constant
amount of lipoprotein lipase, the rate of lipolysis did not differ in
normal and in these hypertriglyceridemic subjects. Furthermore, there was
no correlation between the rates of hydrolysis and the plasma levels of
triglyceride or high density lipoprotein cholesterol.
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