Journal of Lipid Research, Vol 22, 528-531, Copyright © 1981 by Lipid Research, Inc.
A new method for the measurement of bile acid turnover and pool size by a double label, single intubation technique
G Vantrappen, P Rutgeerts and Y Ghoos
A new method is described for measuring the cholic acid turnover and pool
size by a single duodenal intubation technique. The method is based on
determination in a single bile sample of the ratio of the specific
activities of [14C]cholic acid and [3H]cholic acid administered
intravenously with an interval of 24 hr. With this ratio the fractional
turnover rate (k) of cholic acid can easily be calculated as well as the
half-life and pool size. Studies in ten normal subjects indicate that the
cholic acid half life and pool size, determined by this single intubation
technique, correlate very well (r greater than or equal to 0.98) with the
results obtained by Lindstedt's method. Unlike the other methods using a
single intubation, this method allows a good estimate of the bile acid
turnover as well as the bile acid pool size.
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