Journal of Lipid Research, Vol 22, 620-631, Copyright © 1981 by Lipid Research, Inc.
Measurement of bile acid production in hyperlipidemic man: does phenotype or methodology make the difference?
NO Davidson, P Samuel, S Lieberman, SP Shane, JR Crouse and EH Ahrens Jr
Bile acid production has been measured in 13 studies in 10 hyperlipidemic
subjects by simultaneous use of isotope dilution kinetics and chemical
balance methodology. When the data of all 13 studies were averaged, the
correlation between the two sets of values was negative and weak (r - 0.01,
NS). However, when the correlations for normoglyceridemic and
hyperglyceridemic subjects were examined separately, a strong positive
correlation was found between the values obtained by the two methods in
normoglyceridemic subjects (r + 0.92, P less than 0.01) but not in
hyperglyceridemic subjects (r - 0.25, NS). Examination of bile acid
specific activity decay characteristics in eight studies, where bile was
sampled up to six times within the first 24 hr after radio-labeled bile
acid infusion, revealed differences in three rate of attainment of peak
specific activity and in the time taken subsequently to achieve first order
kinetic decay. However, analysis of the data from these eight studies by
input-output analysis yielded values for primary bile acid synthesis no
different that those generated by conventional isotope dilution kinetics.
Thus, bile acid production in normoglyceridemic subjects may be accurately
quantitated by either isotope dilution or chemical balance methodology. Our
data, as well as results from other laboratories, indicate that the values
obtained are strictly comparable. On the other hand, the quantitation of
bile acid production in hyperglyceridemic subjects by isotope dilution
kinetics gives higher values than those obtained by chemical balance
methodology, and in addition, higher values than those obtained in patients
with normal plasma triglyceride levels.
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