Journal of Lipid Research, Vol 22, 705-711, Copyright © 1981 by Lipid Research, Inc.
Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns
G Lepage, CC Roy and AM Weber
Methods currently in use for the quantitative measurement of sulfated serum
bile acids usually lead to low recoveries. The present technique uses two
internal standards and involves enzymatic hydrolysis followed by extraction
of the non-sulfated fraction in ether at pH 4.6. The sulfated fraction is
then solvolysed before gas-liquid chromatographic analysis of the
individual bile acids of both fractions. Using 200 microliters of serum,
recoveries of more than 81% of sulfated bile acids were achieved. This
method is of particular significance in view of recent evidence showing
that certain sulfated bile acids are cholestatic.
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