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Journal of Lipid Research, Vol. 22, 714-719, May 1981
Copyright © 1981 by Lipid Research, Inc.

Separation of molecular species of sphingomyelin and ceramide by argentation and reversed-phase HPLC

Melissa Smith , Peter Monchamp , and Firoze B. Jungalwala

Department of Biochemistry, Eunice Kennedy Shriver Center for Mental Retardation, Waltham MA 02254 and Department of Neurology, Harvard Medical School, Boston MA 02114

A convenient method for the separation of molecular species of complex lipids such as sphingomyelin and ceramide by argentation high performance liquid chromatography (HPLC) is described for the first time. Molecular species separation of these complex lipids was achieved for quantitative analysis and preparative work by the combined use of argentation and reversed-phase HPLC.—Smith, M., P. Monchamp, and F. B. Jungalwala. Separation of molecular species of sphingomyelin and ceramide by argentation and reversed-phase HPLC.

Supplementary key words sphingomyelin • nonhydroxy fatty acid-containing ceramide • hydroxy fatty acid-containing ceramide • benzoylation of sphingomyelin • 3-O-benzoylated ceramide

Submitted on September 18, 1980
Revised on December 18, 1980


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