Journal of Lipid Research, Vol 22, 725-729, Copyright © 1981 by Lipid Research, Inc.
Isotope derivative assay of human serum bile acids
JF Pageaux, B Duperray, M Dubois and H Pacheco
A new method for the selective determination of the main serum bile acids
has been developed. Serum samples with added 14C-labeled bile acid were
submitted to deproteinization, alkaline hydrolysis, methylation, and were
then chromatographed on alumina before acetylation with 2 microliters of
[3H]acetic anhydride. Excess reagent was eliminated by evaporation;
elimination of residual tritiated contaminants and separation of the doubly
labeled bile acid derivatives were obtained by thin-layer chromatography,
column chromatography on Lipidex 5000, and crystallization. The sensitivity
of the method is about 10 pmol of each bile acid. Analyses of seven sera
with normal or elevated concentration of bile acids by the proposed method
and gas- liquid chromatography showed a close correlation (r = 0.94; slope
= 0.93).
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
