Journal of Lipid Research, Vol 22, 735-743, Copyright © 1981 by Lipid Research, Inc.
In vitro transformation of chenodeoxycholic acid and ursodeoxycholic acid by human intestinal flora, with particular reference to the mutual conversion between the two bile acids
S Hirano, N Masuda and H Oda
Nine fecal samples from four healthy subjects were examined for their
ability to transform chenodeoxy-cholic acid (CDCA) and ursodeoxycholic acid
(UDCA) in in vitro anaerobic broth cultures. Seven samples converted CDCA
and UDCA into each other (more than 50% of CDCA was converted into UDCA
while 10% or less of UDCA was converted into CDCA), and produced
7-keto-lithocholic acid and lithocholic acid equally from both acids. No
alteration of the 7 beta-hydroxy group of UDCA was demonstrated by two
fecal samples that failed to perform mutual 7- epimerization, suggesting
the conversion of UDCA into lithocholic acid via CDCA. The 3 alpha-hydroxy
groups of these substrate and metabolite bile acids were invariably
partially epimerized to 3 beta-hydroxy groups by all the fecal samples.
Evidence is presented for the prevalence of these 7- and 3-epimerizing
organisms among the human intestinal flora.
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