Journal of Lipid Research, Vol 22, 811-819, Copyright © 1981 by Lipid Research, Inc.
Circadian variation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in swine liver and ileum
DH Rogers, DN Kim, KT Lee, JM Reiner and WA Thomas
The temporal variation of HMG-CoA reductase activity in the liver and
intestine of swine was investigated. The thin-layer chromatographic method
widely used in the assay of the reductase was successfully applied to the
porcine enzymes. Parallel circadian rhythms were demonstrated in both
hepatic and ileal reductases from mash-fed animals. Peak activity occurred
approximately 6 hr after feeding, 2.7- fold over the basal level in the
liver, and 1.6-fold in the ileum. A milk-cholesterol diet caused a marked
depression of both rhythms (90% in liver, 50% in ileum); however, the
hourly variation in activity persisted in both organs. Cholestyramine was
found to elevate hepatic activity (2.7-fold throughout the rhythm) without
affecting that of the intestine. Clofibrate had no effect on either enzyme
at any time during the cycle despite a 34% reduction in serum cholesterol
concentrations.
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