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Journal of Lipid Research, Vol 22, 877-879, Copyright © 1981 by Lipid Research, Inc.
ARTICLES |
P Kanda and MA Wells
A simplified procedure for the synthesis of short acyl chain phosphatidylcholines is described. A mixed fatty acid-trifluoroacetic anhydride is used to acylate sn-glycero-3-phosphocholine (GPC) which has been dissolved in trifluoroacetic acid. Yields exceeding 70% are achieved in 30-min reaction time using 1.5 to 2.0 equivalents of mixed anhydride per, GPC hydroxyl. This allows a more economical use of labeled short chain acids.
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