Journal of Lipid Research, Vol 22, 882-886, Copyright © 1981 by Lipid Research, Inc.
Quantification of lysophosphatidylethanolamine in the nanomole range
PC Schmid, DR Pfeiffer and HH Schmid
A microanalytical procedure for the determination of trace amounts of
lysophosphatidylethanolamine and other amine-containing lipids is
described. The technique involves reaction of lipid extracts with
fluorescamine to give fluorescent derivatives of lipids containing a free
amino group, separation of the products by thin-layer chromatography, and
quantification by fluorescence spectroscopy after elution from the
adsorbent. Amounts of 100 pmol are easily determined, even in the presence
of large amounts of other phospholipids, and 30-40 samples can be analyzed
per day. Use of the procedure is illustrated with rat liver mitochondria
undergoing a phospholipase A2-dependent increase in inner membrane
permeability due to treatment with Ca2+ plus N-ethylmaleimide.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
