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Journal of Lipid Research, Vol 22, 961-970, Copyright © 1981 by Lipid Research, Inc.

ARTICLES

The biosynthesis, absorption, and origin of cholesterol and plant sterols in the Florida land crab

TS Douglass, WE Connor and DS Lin

In order to study the biosynthesis, composition, and origin of sterols in the Florida land crabs, Cardisoma guanhumi (Latreille), we fed 17 male crabs either a cholesterol-free or a high cholesterol diet for 2 to 7 weeks. The origin of sterols in these crabs, whether from biosynthesis or from the diet, was determined by tahree procedures: the incorporation of isotopic mevalonate into the cholesterol when the diet was cholesterol-free; the absorption of isotopic cholesterol and sitosterol from the diet; the cholesterol and plant sterol concentrations of hepatopancreas, plasma, and muscle under conditions of cholesterol-free and high cholesterol diets. In addition, the interconversion of cholesterol and sitosterol was investigated. Dietary sterols of plant and animal sources were readily absorbed and provided the major source of sterols for this species of crab. The biosynthesis of cholesterol from mevalonate in this crab was minimal. However, cholesterol was synthesized from dietary sitosterol by dealkylation. Cholesterol and the three plant sterols (24 epsilon-methyl cholesterol, stigmasterol, and sitosterol) were found in the hepatopancreas, plasma, and muscle of the crab. Plant sterols contributed from 9 to 37% of the total sterols in the hepatopancreas, plasma, and muscle of the crabs fed a cholesterol-free diet.
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