Journal of Lipid Research, Vol 22, 1094-1101, Copyright © 1981 by Lipid Research, Inc.
Quantitative changes in adipocyte plasma membrane in response to nutritional manipulations
DS Lewis, EJ Masoro and BP Yu
The effects of changes in adipocyte size and the effects of nutritional
manipulations on the quantity of plasma membrane per adipocyte were
investigated. A method for estimating the quantity of plasma membrane was
developed based on the specific labeling of adipocyte plasma membrane
protein with the nonpermeable labeling agent 125I-labeled diazotized
diiodosulfanilic acid. By studying rats (ranging in age from 50 to 125
days) fed a standard laboratory chow or a low fat diet or a high fat diet,
a wide range of mean fat cell sizes was obtained. It was found that as the
volume of the fat cell increased, the amount of plasma membrane increased
in a linear fashion and that this linear relationship had the same slope
whether the size of the adipocyte increased slowly with age or rapidly in
response to a high fat diet. In contrast, fasting for up to 3 days caused a
marked decrease in the mean volume of the adipocytes, but either no change
or much less change in the amount of plasma membrane per cell than would
have been predicted from the linear relationship between adipocytes, but
either no change or much less change in the amount of plasma membrane per
cell than would have been predicted form the linear relationship between
adipocyte volume and amount of plasma membrane per cell obtained with fed
rats, i.e., adipocytes from fasted rats contain more plasma membrane per
cell than do fat cells of the same size from fed rats. Neither feeding a
high fat diet nor fasting caused detectable changes in the protein and
lipid composition of the adipocyte plasma membrane.
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