HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Albers, J. J. Articles by Adolphson, J. L. Search for Related Content PubMed PubMed Citation Articles by Albers, J. J. Articles by Adolphson, J. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 22, 1206-1213, November 1981
Copyright © 1981 by Lipid Research, Inc.

Lecithin:cholesterol acyltransferase (LCAT) mass; its relationship to LCAT activity and cholesterol esterification rate

John J. Albers , Ching Hong Chen , and Janet L. Adolphson

Department of Medicine, Northwest Lipid Research Clinic, Harborview Medical Center, University of Washington School of Medicine, Seattle, WA

The relationship between plasma lecithin:cholesterol acyltransferase mass and enzyme activity and between mass and plasma cholesterol esterification rate was determined in 25 adult volunteers without overt disease (14 normolipidemic and 11 hyperlipidemic). Furthermore, the relationship of lecithin:cholesterol acyltransferase mass and cholesterol esterification rate to lipids, apoproteins, age, and ideal body weight was assessed. Lecithin:cholesterol acyltransferase mass determined by radioimmunoassay was highly correlated with enzyme activity assayed using a heated plasma substrate (r = 0.636) and with the molar cholesterol esterification rate determined either by radioassay (r = 0.809) or by measurement of the decrease of unesterified cholesterol (r = 0.621). Lecithin:cholesterol acyltransferase mass was also positively correlated with total cholesterol (r = 0.608), unesterified cholesterol (r = 0.562), age (r = 0.544), and percent ideal body weight (r = 0.619), but was not significantly correlated with log triglyceride, high density lipoprotein cholesterol, or apolipoproteins A-I, A-II, or D. Plasma cholesterol esterification rate by both methods was highly positively correlated with total cholesterol, unesterified cholesterol, log triglyceride, and age, but was inversely correlated with high density lipoprotein cholesterol. Upon partial correlation analysis with lecithin:cholesterol acyltransferase mass kept constant the cholesterol esterification rate remained significantly positively related to total cholesterol, unesterified cholesterol, and log triglyceride and inversely related to high density lipoprotein cholesterol. Two subjects had normal lecithin:cholesterol acyltransferase but approximately half normal molar cholesterol esterification rate. Measurement of lecithin:cholesterol acyltransferase mass and activity along with plasma cholesterol esterification rate will permit differentiation of abnormalities of enzyme from qualitative or quantitative substrate or cofactor abnormalities. Also, the finding that the regression line between LCAT mass and the plasma esterification rate by direct determination of unesterified cholesterol passes through the origin suggests that all immunodetectable LCAT in plasma is active in normal subjects.—Albers, J. J., C-H. Chen, and J. L. Adolphson. Lecithin:cholesterol acyltransferase (LCAT) mass; its relationship to LCAT activity and cholesterol esterification rate.

Supplementary key words radioimmunoassay • enzyme • lipids • lipoproteins

Submitted on February 13, 1981
Revised on June 8, 1981

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J.-Y. Lee, R. M. Badeau, A. Mulya, E. Boudyguina, A. K. Gebre, T. L. Smith, and J. S. Parks Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice J. Lipid Res., May 1, 2007; 48(5): 1052 - 1061. [Abstract] [Full Text] [PDF]

				J. Q. Zhang, B. Smith, M. M. Langdon, H. L. Messimer, G. Y. Sun, R. H. Cox, M. James-Kracke, and T. R. Thomas Changes in LPLa and reverse cholesterol transport variables during 24-h postexercise period Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E267 - E274. [Abstract] [Full Text] [PDF]

				K. Kobori, K. Saito, S. Ito, K. Kotani, M. Manabe, and T. Kanno A new enzyme-linked immunosorbent assay with two monoclonal antibodies to specific epitopes measures human lecithin-cholesterol acyltransferase J. Lipid Res., February 1, 2002; 43(2): 325 - 334. [Abstract] [Full Text] [PDF]

				S. C. Riemens, A. Van Tol, W. J. Sluiter, and R. P. F. Dullaart Acute and chronic effects of a 24-hour intravenous triglyceride emulsion challenge on plasma lecithin: cholesterol acyltransferase, phospholipid transfer protein, and cholesteryl ester transfer protein activities J. Lipid Res., August 1, 1999; 40(8): 1459 - 1466. [Abstract] [Full Text]

				T. V. Fungwe, B. J. Kudchodkar, A. G. Lacko, and L. Dory Fatty Acids Modulate Lecithin:Cholesterol Acyltransferase Secretion Independently of Effects on Triglyceride Secretion in Primary Rat Hepatocytes J. Nutr., August 1, 1998; 128(8): 1270 - 1275. [Abstract] [Full Text]

				B. Garner, A. R. Waldeck, P. K. Witting, K.-A. Rye, and R. Stocker Oxidation of High Density Lipoproteins. II. EVIDENCE FOR DIRECT REDUCTION OF LIPID HYDROPEROXIDES BY METHIONINE RESIDUES OF APOLIPOPROTEINS AI AND AII J. Biol. Chem., March 13, 1998; 273(11): 6088 - 6095. [Abstract] [Full Text] [PDF]

				N. Sakai, B. L. Vaisman, C. A. Koch, R. F. Hoyt Jr., S. M. Meyn, G. D. Talley, J. A. Paiz, H. B. Brewer Jr., and S. Santamarina-Fojo Targeted Disruption of the Mouse Lecithin:Cholesterol Acyltransferase (LCAT) Gene. GENERATION OF A NEW ANIMAL MODEL FOR HUMAN LCAT DEFICIENCY J. Biol. Chem., March 14, 1997; 272(11): 7506 - 7510. [Abstract] [Full Text] [PDF]

				J. Hasler-Rapacz, H. J. Kempen, H. M.G. Princen, B. J. Kudchodkar, A. Lacko, and J. Rapacz Effects of Simvastatin on Plasma Lipids and Apolipoproteins in Familial Hypercholesterolemic Swine Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 137 - 143. [Abstract] [Full Text]

				T. Murakami, S. Michelagnoli, R. Longhi, G. Gianfranceschi, F. Pazzucconi, L. Calabresi, C. R. Sirtori, and G. Franceschini Triglycerides Are Major Determinants of Cholesterol Esterification/Transfer and HDL Remodeling in Human Plasma Arterioscler. Thromb. Vasc. Biol., November 1, 1995; 15(11): 1819 - 1828. [Abstract] [Full Text]

				H.-G. Klein, N. Duverger, J. J. Albers, S. Marcovina, H. B. Brewer Jr., and S. Santamarina-Fojo In Vitro Expression of Structural Defects in the Lecithin-Cholesterol Acyltransferase Gene J. Biol. Chem., April 21, 1995; 270(16): 9443 - 9447. [Abstract] [Full Text] [PDF]