Journal of Lipid Research, Vol 23, 211-215, Copyright © 1982 by Lipid Research, Inc.
Drawbacks in the use of 23-nor-deoxycholic acid standards
AF Attili, M Angelico, L Capocaccia, U Pieche and A Cantafora
23-Nor-deoxycholic acid is widely used as internal standard in gas- liquid
chromatographic studies of bile acids. Two batches of this compound,
submitted to conventional alkaline hydrolysis of bile acid conjugates, were
found to be transformed into a product with chromatographic properties
different from those of "authentic" 23-nor- deoxycholic acid. To identify
this "new" product a comparison was made between chromatographic
properties, mass spectra, and NMR spectra of 23- nor-deoxycholic acid
before and after alkaline hydrolysis. The results indicate that the "new"
compound is the true 23-nor-deoxycholic acid while the product present in
the two batches examined is a monoacetate derivative.
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