Journal of Lipid Research, Vol 23, 299-306, Copyright © 1982 by Lipid Research, Inc.
Biochemical and ultrastructural alterations in platelets, reticulocytes, and lymphocytes from rats fed vitamin E-deficient diets
J Lehmann and M McGill
Effects of vitamin E-deficiency, dietary fat (corn oil versus lard), and
incubation on ultrastructure of platelets, red and white cells, and on
selected biochemical parameters of platelets and plasma were studied.
Platelets from vitamin E-deficient rats had no obvious morphological
defects, but reticulocytes and lymphocytes had swollen and deformed
mitochondria. Fatty acid and glycogen levels of platelets were not affected
by the deficiency but total lipid levels in plasma were decreased of
increased depending upon the type and level of dietary fat. In comparison
with supplemented controls, the proportion of stearate increased in the
phospholipid fraction of plasma from vitamin E-deficient, lard-fed but not
corn oil-fed rats. In platelets, total fatty acids per mg protein were
8-12% lower with lard than with corn oil as fat source. Oleate and
linoleate were higher and lower, respectively, although not to the same
degree, and arachidonate was not affected. With incubation of platelet-rich
plasma at 37 degrees C for 6 hr, there were no obvious morphological
changes in platelets from control or from deficient, lard-fed rats, but
platelets from deficient, corn oil-fed rats contained mitochondria that
were swollen and deformed. Incubation did not affect fatty acid, glycogen,
or tocopherol levels of platelets or tocopherol levels of plasma,
regardless of type or amount of dietary fat.
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