Journal of Lipid Research, Vol 23, 357-359, Copyright © 1982 by Lipid Research, Inc.
Loss of human serum apolipoproteins C and E during manipulation of diluted solutions
L Holmquist
The adsorption of human serum very low density apolipoproteins C-I, C- II,
C-III, and E to the interior surfaces or Beckman Airfuge plastic tubes and
glass pipettes has been studied. 125I-Labeled apolipoprotein C-III-1 (1
microgram/ml) was used as a model compound to study the adsorption. After
60 min about 45% was bound to the tube walls, and 5- 10% bound rapidly to
the pipette used for removing the solution from the tube. Addition of
increasing amounts of non-labeled apolipoprotein C-I, C-II, C-III, or E to
the tubes reduced the amount of labeled C-III on the tube walls, indicating
a strong affinity of these apolipoproteins for the surface. The results
demonstrate the importance of careful control of the different steps in
immunological ultramicroquantitation methods, that might be influenced by
adsorption- losses of the apolipoproteins.
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