Journal of Lipid Research, Vol 23, 360-363, Copyright © 1982 by Lipid Research, Inc.
Improved insulin responsiveness in rat adipose tissue pieces cultured with charcoal-treated albumin and oxygen
RS Bernstein
In an effort tao simulate the effects of insulin on fat cells prepared from
fresh adipose tissue, pieces of epididymal adipose tissue were cultured in
a medium containing charcoal-treated bovine serum albumin (BSA) with a gas
phase of 100% O2. Using this system, the insulin effect on [1-14C]glucose
oxidation was retained, in contrast to previous results in culture with
untreated BSA in room air. Basal [1- 14C]glucose oxidation was similar to
fresh tissue, and insulin stimulated oxidation by 137%. In contrast to the
effects of this culture system on [1-14C]glucose oxidation, tissue cultured
with charcoal-treated BSA had lower basal rates of [U-14C]glucose
utilization and 2-deoxyglucose uptake than either cells from fresh tissue
or from tissue cultured with untreated BSA. The insulin effect on both of
these measures was similar for the two culture systems and lower than for
fresh tissues. Rates of lipolysis were increased in both types of cultured
fat cells. Thus the improvement in [1-14C]glucose oxidation is presumably
an effect on the pentose phosphate shunt, does not reflect a change in
glucose transport or overall glucose utilization, and is not caused by a
reduction in free fatty acid levels.
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