Journal of Lipid Research, Vol 23, 422-427, Copyright © 1982 by Lipid Research, Inc.
Changes in the metabolism of fatty acids in adipose tissue in obese patients with primary hypertriacylglycerolemia
P Arner, P Engfeldt and J Ostman
Changes in the release and esterification of free fatty acids (FFA) in
adipose tissue were looked for as a cause of moderate primary
hypertriacylglycerolemia (HTG) in five obese subjects. Comparison was made
with six obese normolipidemic subjects. The two groups were matched for
body weight, tolerance of intravenous glucose, fat cell size, fasting
levels of serum immunoreactive insulin, and serum insulin response to an
intravenous glucose load. Subcutaneous adipose tissue was incubated in
vitro with [1-14C]palmitic acid for 30, 60, and 120 min. There was a
significant, twofold increase in the rate of FFA mobilization, but no
change of glycerol release in HTG patients. The adipose tissue levels of
mono- and diacylglycerols were similar in the two groups of subjects and
did not change during incubation. Re- esterification of FFA, calculated
from the net changes in medium and in tissue FFA and glycerol release, was
lower in HTG patients than in the controls (3 and 12 mumol/10(7) cells/hr,
respectively; P less than 0.025). In adipose tissue of HTG patients, the
amount of radioactive fatty acids incorporated into triacylglycerols (TG)
was 50% lower (P less than 0.025), whereas that incorporated into tissue
FFA was three times higher (P less than 0.01) when compared with control
patients. It is concluded that, in adipose tissue of obese patients with
primary hypertriacylglycerolemia, the esterification of free fatty acids to
triacylglycerol is decreased. As a consequence, free fatty acids are
mobilized at an increased rate.
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