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Journal of Lipid Research, Vol 23, 502-507, Copyright © 1982 by Lipid Research, Inc.

ARTICLES

Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples

PF Bougneres and DM Bier

In studies where [1-13C]palmitic acid is employed as isotopic tracer in vivo, we have described a selected ion monitoring gas-liquid chromatography-mass spectrometry micro-method which allows plasma tracer enrichment as well as plasma palmitate content to be determined in the same 100-microliter sample through the use of [5,5,6,6- 2H4]palmitic acid as assay internal standard. For standard solutions in the range equivalent to plasma palmitic acid concentrations of 10--2500 microM, assay precision was +/- 5%. For plasma samples in the physiologic range (approximately 30--200 microM palmitate) assay precision averaged better than +/- 2%. The use of the method is illustrated by measuring palmitate turnover in a 12-hr-old human infant.
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