Journal of Lipid Research, Vol 23, 653-659, Copyright © 1982 by Lipid Research, Inc.
Human plasma lipid exchange protein(s): a method for separation of donor and acceptor lipoproteins by heparin-Sepharose chromatography
JL Ellsworth, L McVittie and RL Jackson
The transfer or exchange of cholesteryl esters, triglycerides, and
phospholipids between plasma very low (VLDL), low (LDL), and high (HDL)
density lipoproteins is facilitated by specific lipid transfer proteins.
The present report describes a method to separate donor and acceptor
lipoprotein pools used in assays for lipid exchange activities. The method
is based on the differential binding of lipoproteins to immobilized
heparin. At 50 mM NaCl concentration, VLDL and LDL bind to
heparin-Sepharose whereas greater than 85% of HDL is unretained; VLDL and
LDL are than eluted with 300 mM NaCl, 2% sodium dodecyl sulfate with a
recovery greater than 85%. The procedure is rapid and quantitative, as
judged by a comparison to ultracentrifugation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
