Journal of Lipid Research, Vol 23, 823-830, Copyright © 1982 by Lipid Research, Inc.
Bile acids and lipids in isolated rat hepatocytes: content, synthesis, and release, as affected by cholestyramine treatment of the donor rats
HJ Kempen, MP Vos-Van Holstein and J de Lange
Contents of bile acids and lipids, as well as rates of triglyceride
synthesis, were determined in isolated hepatocytes from control or
cholestyramine-fed rats (denoted below as "control" or "treated"
hepatocytes, respectively). During a 3-hr incubation period, total bile
acid production was markedly higher in "treated" cells than in "control"
cells. With both kinds of cells a marked fall in production rate occurred
after the first hour of incubation. Newly produced bile acids appeared in
the conjugated form with both kinds of hepatocytes. "Control" cells
produced only taurine-conjugated, while "treated" cells made both
taurine-conjugated and glycine-conjugated bile acids. However, with
exogenous taurine (0.5 mM), the latter cells also produced
taurine-conjugated bile acids only. With both kinds of cells, cholic and
beta-muricholic acids, but not dihydroxylated bile acids, appeared as newly
formed species during the incubation. Addition of dialyzed rat serum to the
incubation did not result in a stimulation of bile acid production, with
either kind of hepatocytes. "Treated" cells had a slightly higher content
of free cholesterol than control cells; contents of other lipids were not
different. Fractional release of bile acids and lipids into the medium did
not differ between the two kinds of cells. Triglyceride synthesis from
added [14C]palmitate (0.5 mM) was 1.8-fold higher in "treated" than in
"control" hepatocytes.
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