J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wieland, H.
Right arrow Articles by Seidel, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wieland, H.
Right arrow Articles by Seidel, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol 23, 893-902, Copyright © 1982 by Lipid Research, Inc.

ARTICLES

Determination of apolipoprotein B by kinetic (rate) nephelometry

H Wieland, P Cremer and D Seidel

Determination of apoB by rate nephelometry is a simple, rapid and precise method, which is also suitable for a routine laboratory. It can be standardized with lipoprotein-B or LDL fractions, but only if these fractions are diluted with fresh whole serum. The standardization is also valid for VLDL if the samples are diluted with hydroxypolyethoxydodecane (trade name: Thesit) in a concentration of 0.33 g/liter. Using rate nephelometry, a strong correlation between the contents of cholesterol and apoB of VLDL as well as of LDL can be demonstrated. Similar high correlations are achieved if apoB is determined chemically in isolated lipoprotein fractions. The ratio of apoB to cholesterol is constant but not the same in both VLDL and LDL. There is also a strong correlation between the concentration of apoB in whole serum with the LDL cholesterol values in a normotriglyceridemic population. Therefore, the assay of apoB in whole serum by rate nephelometry is proposed as a screening method for dyslipoproteinemia (increased concentrations of atherogenic lipoproteins at normal or elevated levels of cholesterol in plasma).
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
E. Campos, L. Kotite, P. Blanche, Y. Mitsugi, P. H. Frost, U. Masharani, R. M. Krauss, and R. J. Havel
Properties of triglyceride-rich and cholesterol-rich lipoproteins in the remnant-like particle fraction of human blood plasma
J. Lipid Res., March 1, 2002; 43(3): 365 - 374.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. N. Duchateau, C. R. Pullinger, R. E. Orellana, S. T. Kunitake, J. Naya-Vigne, P. M. O'Connor, M. J. Malloy, and J. P. Kane
Apolipoprotein L, a New Human High Density Lipoprotein Apolipoprotein Expressed by the Pancreas. IDENTIFICATION, CLONING, CHARACTERIZATION, AND PLASMA DISTRIBUTION OF APOLIPOPROTEIN L
J. Biol. Chem., October 10, 1997; 272(41): 25576 - 25582.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1982 by the American Society for Biochemistry and Molecular Biology.