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Journal of Lipid Research, Vol. 23, 1032-1038, September 1982
Copyright © 1982 by Lipid Research, Inc.

Antigenic mapping of human low density lipoprotein with monoclonal antibodies

M. J. Tikkanen , R. Dargar , B. Pfleger , B. Gonen , J. M. Davie , and G. Schonfeld

Lipid Research Center, Departments of Preventive Medicine, Medicine, Microbiology, and Immunology, Washington University School of Medicine, St. Louis, MO 63110

Monoclonal anti-LDL antibodies were produced in a mouse spleen-myeloma system and purified by affinity chromatography on insolubilized low density lipoprotein (LDL). Five antibodies with different specificities could be distinguished by their immunoreactivities with chemically modified LDL preparations, and by their competition for binding to LDL. One of the antibodies inhibited the binding of ¹²⁵I-labeled LDL to the apoB,E receptors of cultured human fibroblasts. The same degree of inhibition was achieved using isolated Fab fragments. This antibody may bind to an antigenic site located near the cellular binding site of LDL-apoB.—Tikkanen, M. J., R. Dargar, B. Pfleger, B. Gonen, J. M. Davie, and G. Schonfeld. Antigenic mapping of human low density lipoprotein with monoclonal antibodies.

Supplementary key words fibroblasts • apoB,E receptors • modified LDL

Submitted on February 1, 1982
Revised on April 30, 1982

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