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Journal of Lipid Research, Vol 23, 1072-1075, Copyright © 1982 by Lipid Research, Inc.
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A simple procedure suitable for rapid transmethylation of triacylglycerols, other neutral lipids (including cholesteryl esters), and glycerophospholipids is described. Lipids in diethyl ether solution (50 volumes), in the presence of methyl acetate (1 vol), are reacted with 1 M sodium methoxide in methanol (1 vol) at room temperature. Essentially complete transmethylation can occur within a few minutes with no hydrolysis. Glassware and reagent requirements are minimal and samples are ready for gas-liquid chromatography analysis with very little work-up.
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P. Y. Chouinard, L. Corneau, D. M. Barbano, L. E. Metzger, and D. E. Bauman Conjugated Linoleic Acids Alter Milk Fatty Acid Composition and Inhibit Milk Fat Secretion in Dairy Cows J. Nutr., August 1, 1999; 129(8): 1579 - 1584. [Abstract] [Full Text]
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M. L. Kelly, J. R. Berry, D. A. Dwyer, J.M. Griinari, P. Y. Chouinard, M. E. Van Amburgh, and D. E. Bauman Dietary Fatty Acid Sources Affect Conjugated Linoleic Acid Concentrations in Milk from Lactating Dairy Cows J. Nutr., May 1, 1998; 128(5): 881 - 885. [Abstract] [Full Text]
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