Journal of Lipid Research, Vol 23, 972-983, Copyright © 1982 by Lipid Research, Inc.
Enrichment of the intracellular dolichol pool in isolated liver cells
T Ekstrom, T Chojnacki and G Dallner
Isolated hepatocytes were incubated with egg lecithin liposomes containing
dolichol(C55), dolichol(C95), and dolichol phosphate(C55) in order to
enrich intracellular membranes with these polyprenols. After incubation,
the lipids were recovered from various membrane fractions and from the
supernatant. The highest concentration was found in the microsomes. A part
of the dolichol in microsomes, as well as in other fractions, was
phosphorylated. This phosphorylation is mediated by the CTP-specific kinase
that is present only on the outer surface of the microsomes and uses
alpha-saturated polyprenols as substrates. The isolated microsomes enriched
with dolichol in vivo exhibited increased lipid and protein glycosylation
upon incubation with nucleotide sugars and it was demonstrated that the
increased lipid glycosylation was due to transfer of the sugar to the
exogenous incorporated dolichol.
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