Journal of Lipid Research, Vol 23, 1143-1151, Copyright © 1982 by Lipid Research, Inc.
Studies on fat digestion, absorption, and transport in the suckling rat. III. Composition of bile and evidence for enterohepatic circulation of bile salts
JE Staggers, SC Frost and MA Wells
Biliary secretions from suckling rats (10-15 days old) were characterized:
bile flow rate was 197.3 microliters/hr per 100 g; bile salt pool size was
19.0 mumol/100 g; secretion rate was 14.5 mumol/hr per 100 g; synthesis
rate was 12.0 mumol/day; and the daily turnover frequency was 18.3.
Phospholipid and cholesterol secretion rates were 2.3 mumol/hr per 100 g
and 0.17 mumol/hr per 100 g, respectively. The bile salt concentration in
portal plasma was 0.17 mumol/ml. The fatty acid composition of biliary
phosphatidylcholine and ethanolamine, as well as the stereospecific
distribution of fatty acids in the former, were similar to that found in
phospholipids from adult rat bile. Compositional analysis of bile acids
showed greater than 98% taurine conjugates consisting of approximately 54%
cholic acid, 40% beta- muricholic acid, 2% chenodeoxycholic acid, and 1%
each of deoxycholic and hyodeoxycholic acids. Through the use of intestinal
and liver perfusion experiments, we have obtained evidence for
enterohepatic circulation of taurocholate in neonatal rats. The level of
bile salts found in intestinal contents (49.5 mumol/g), and the biliary
phospholipid concentration (11.8 mM) both exceed adult values (6-10 mumol/g
and 6.3 mM, respectively) and may be important for the utilization of the
large amount of milk triacylglycerols ingested during the suckling period.
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