Journal of Lipid Research, Vol 23, 1167-1173, Copyright © 1982 by Lipid Research, Inc.
Hepatic bile acid uptake: effect of conjugation, hydroxyl and keto groups, and albumin binding
R Aldini, A Roda, AM Labate, G Cappelleri, E Roda and L Barbara
Hepatic extraction of trihydroxy (free, glyco- and tauro-conjugated)
dihydroxy, and monohydroxy bile acids has been evaluated in single pass
liver perfusion experiments in rats. The percentage of each bile acid bound
to albumin was also evaluated by equilibrium dialysis. Conjugation
increased bile acid liver extraction, without relevant differences in the
percentage of bile acid bound to albumin. Among the free bile acids,
trihydroxy bile acids were more efficiently cleared by the liver than the
dihydroxy acids, and the latter more than monohydroxy bile acids.
7-Ketolithocholic acid uptake was slightly less than that of cholic acid.
Conversely, among dihydroxy bile acids, the percentage of the bile acid
bound to albumin decreased from lithocholic acid to cholic acid. Decreasing
the albumin concentration in the medium, and hence the fraction of the bile
acid bound to albumin, resulted in an increase in bile acid liver
extraction. Therefore besides differences in the chemical structure of bile
acids, the extent of bile acid-albumin binding may be a determinant in bile
acid liver uptake.
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