Journal of Lipid Research, Vol 23, 1246-1248, Copyright © 1982 by Lipid Research, Inc.
Synthesis of 14C-labeled alkyl polyoxyethylene detergents
G Fredrikson, L Krabisch and P Belfrage
Procedures for the synthesis and purification of two 14C-labeled alkyl
polyoxyethylene detergent preparations are described. One of the
preparations consisted mainly of [14C]dodecyloctaoxyethylene ether, the
other consisted mainly of [14C] octylhexaoxyethylene ether, with critical
micellar concentrations of approximately 50 microM and 10 mM, respectively.
Condensation of alkyl bromides with fractionated polyethyleneglycol
resulted in less polydisperse products than conventional ethylene oxide
condensation. Highly purified detergent species could easily be obtained
from the preparations by thin-layer chromatography. Their use as tracers
greatly simplifies determination of detergent concentration during protein
solubilization and fractionation.
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