Journal of Lipid Research, Vol 23, 1292-1300, Copyright © 1982 by Lipid Research, Inc.
Involvement of triacylglycerol in the metabolism of fatty acids by cultured neuroblastoma and glioma cells
HW Cook, JT Clarke and MW Spence
The metabolism (chain elongation, desaturation, and incorporation into
complex lipids) of thirteen different radiolabeled fatty acids and acetate
was examined in N1E-115 neuroblastoma and C-6 glioma cell lines in culture.
During 6-hr incubations, all fatty acids were extensively (14-80%)
esterified to complex lipids, mainly choline phosphoglycerides and
triacylglycerol. With trienoic and tetraenoic substrates, inositol and
ethanolamine phosphoglycerides also contained up to 30% of the labeled
fatty acids; plasmalogen contained up to half of the label in the
ethanolamine phosphoglyceride fraction of neuroblastoma cells. Chain
elongation and delta 9, delta 6, and delta 5 desaturation occurred in both
cell lines; delta 4 desaturation was not observed. Seemingly anomalous
utilization of arachidic acid and some selectivity based on the geometric
configuration of double bonds was observed. These studies indicate that
these cell lines are capable of modulating cellular membrane composition by
a combination of selective exclusion and removal of inappropriate acyl
chains and of modification of other acyl chains by desaturation and chain
elongation. The time courses and patterns of modification and incorporation
of exogenous substrates into phospholipids and triacylglycerol suggest that
exogenous unsaturated fatty acid may be incorporated into triacylglycerol
and later released for further metabolism and incorporation into
phospholipids. This supports a role for triacylglycerol in the synthesis of
membrane complex lipids in cell lines derived from neural tissue.
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