Journal of Lipid Research, Vol 24, 34-46, Copyright © 1983 by Lipid Research, Inc.
Incorporation of deuterium-labeled trans- and cis-13-octadecenoic acids in human plasma lipids
EA Emken, RO Adlof, WK Rohwedder and RM Gulley
The absorption and distribution of deuterated trans- and cis-13-
octadecenoic acid (13t-18:1 and 13c-18:1) in plasma lipids were compared to
deuterated cis-9-octadecenoic acid (9c-18:1) in two young adult male
subjects. A mixture of triglycerides was fed in a multiple- labeled
experiment where each triglyceride contained a fatty acid labeled with a
different number of deuterium atoms. Analysis of human plasma lipids by
mass spectroscopy allowed the distribution of the two 13-octadecenoic acid
isomers to be directly compared to cis-9- octadecenoic acid. Plasma lipids
selectively excluded both the 13t-18:1 and 13c-18:1 isomers relative to
9c-18:1 in all neutral and phospholipid fractions. Discrimination against
incorporation of the 13t- 18:1 isomer into plasma cholesteryl ester and
2-acyl phosphatidylcholine was nearly absolute. The 1-acyl
phosphatidylcholine fraction exhibited a large positive selectivity for the
13t-18:1 isomer. Differences in the relative distribution of the trans and
cis 13-18:1 isomers vs. 9c-18:1 in the various lipoprotein lipid classes
were found. Analysis of the chylomicron triglyceride component of the
plasma lipids indicated all three fatty acids were equally well absorbed.
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