Journal of Lipid Research, Vol 24, 1485-1493, Copyright © 1983 by Lipid Research, Inc.
Allotypes associated with B apolipoproteins in rabbits
LS Huang, JS Jaeger and DC Usher
Western blot analysis of the alloantisera (i.e., anti-Lpq1, anti-Lpq2,
anti-Lpq3, and anti-Lpq4) which defined the three lpq genes of rabbit
linkage group VIII showed that they reacted strongly with an apolipoprotein
of molecular weight 320,000. They also cross-reacted with an apolipoprotein
of molecular weight 220,000. The two apolipoproteins that reacted with the
alloantisera were found by SDS- polyacrylamide gel electrophoresis to be
present in very low density (VLDL), intermediate density (IDL), and low
density (LDL) lipoprotein fractions and by Western blot analysis to react
with an anti- apolipoprotein B antiserum. These results support the
conclusion that the alloantisera react with allotypes associated with the B
apolipoproteins. The distribution of the four allotypes among different
lipoprotein fractions, however, differed. The quantitative competitive
Enzyme Linked Immunosorbant Assay (ELISA) showed that the Lpq1, Lpq2, and
Lpq4 allotypes were found in the highest concentration in VLDL, IDL, and
LDL, and in significantly lower concentrations in plasma chylomicrons. The
concentrations of these allotypes in high density lipoproteins (HDL) as
measured in the ELISA were about 1% of the concentrations found in LDL. The
Lpq3 allotype, on the other hand, was present in the highest concentrations
only in IDL and LDL and in significantly lower concentrations in VLDL and
plasma chylomicrons. Surprisingly, the concentration of the Lpq3 allotype
in HDL was 20% of the level found in LDL.
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