Journal of Lipid Research, Vol 24, 1646-1651, Copyright © 1983 by Lipid Research, Inc.
Separation of steryl esters by reversed-phase liquid chromatography
JT Billheimer, S Avart and B Milani
Thirty steryl esters composed of ten different fatty acids and 14 sterols
as well as two wax esters were analyzed by reversed-phase liquid
chromatography on a Zorbax ODS column at 205 nm using
acetonitrile-isopropanol 60:40 (v/v) as a mobile phase. Contributions
(sigma values) of individual double bonds and alkyl groups in the steryl
ester were determined from the relative retention values for steryl esters
with and without the feature. A double bond and an alkyl group had a
greater effect on the retention time of a steryl ester when present in the
fatty acid rather than in the sterol moiety. The sigma value can be used to
obtain structural information about a steryl ester. Separation of a complex
mixture of steryl esters found in corn oil was achieved using this
technique.
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