Journal of Lipid Research, Vol 24, 211-215, Copyright © 1983 by Lipid Research, Inc.
Separation of mono-, di-, and trihydroxy stereoisomers of bile acids by capillary gas-liquid chromatography
T Iida, FC Chang, T Matsumoto and T Tamura
Capillary gas-liquid chromatographic separation was studied for the
complete set of the 26 theoretically possible isomers of mono-, di-, and
trihydroxylated 5 beta-cholanic acids, which differ from one another in the
number, position, and configuration of hydroxyl groups at C-3, C-7, and/or
C-12 in the nucleus, as well as for some of their related acids. The bile
acid samples were chromatographed as their methyl ester-trimethylsilyl
(TMSi) ether derivatives and analyzed on three capillary columns coated
with nonpolar OV-1, slightly polar OV- 17, and polar SP-2340 as liquid
phases. The retention times on capillary gas-liquid chromatography (GLC)
responded dramatically to the minor structural differences, and almost
complete separation of the positional and stereochemical isomers was
achieved by the combined use of SP-2340 and OV-17 (or OV-1) capillary
columns.
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