Journal of Lipid Research, Vol 24, 216-220, Copyright © 1983 by Lipid Research, Inc.
Hydrazinolysis of lipids and analysis of their constituent fatty acids
VP Agrawal
Treatment of O-acyl lipids with hydrazine degrades them to hydrazides of
the constituent fatty acids. These hydrazides react with acetone to yield
less polar and more volatile N'-isopropylidenealkanohydrazides, R- CO-NH-N
= C(CH3)2, which strongly absorb ultraviolet light and are amenable to
thin-layer and gas-liquid chromatographic analyses. In presence of
cyclopentene, reduction of double bonds in unsaturated fatty acids with
hydrazine does not occur. The method of hydrazinolysis followed by
acetonization has been used for fatty acid analysis of different classes of
lipids.
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