Journal of Lipid Research, Vol 24, 297-302, Copyright © 1983 by Lipid Research, Inc.
A model for cholesterol absorption: isotope vs. mass; single dose vs. constant infusion
DB Zilversmit
A study is presented to evaluate the relative merits of isotope and
cholesterol mass measurements for cholesterol absorption. In this study,
cholesterol absorption is simulated as a sequence of 10 two-pool segments
in which a concentration gradient of cholesterol mass and/or label exists
between the site of exogenous cholesterol entry and that of fecal loss. The
model is governed by first order rate constants both for label and for
mass. The appearance of labeled cholesterol in lymph and feces provides a
reliable measure of the cholesterol mass increment in lymph due to
exogenous cholesterol absorption. Net cholesterol absorption, calculated
from constant infusion experiments, differs numerically from this mass
increment. A dual isotope fecal ratio method agrees with other labeling
techniques, but gives reliable information only when feces are collected
for a sufficiently long time.
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