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Journal of Lipid Research, Vol 24, 343-356, Copyright © 1983 by Lipid Research, Inc.
Cholesterol absorption and transport in thoracic duct lymph lipoproteins of nonhuman primates. Effect of dietary cholesterol level
RL Klein and LL Rudel
The effect of dietary cholesterol level on cholesterol absorption and its
subsequent transport in thoracic duct lymph lipoproteins was studied in two
species of nonhuman primates, namely the African green monkey
(Cercopithecus aethiops) and the cynomolgus macaque (Macaca fascicularis).
Each animal served as its own control because each received sequential,
intraduodenal infusions of two fat-rich liquid diets that differed only in
the amounts of cholesterol. The percentage of dietary cholesterol absorbed
was calculated by dividing the rate of appearance of exogenous cholesterol
radioactivity in lymph by the rate of infusion of dietary cholesterol
radioactivity at a time when lymph cholesterol specific activity was
constant relative to that of diet. The percentage of dietary cholesterol
absorbed was similar during both diet infusions in African green monkeys
but was significantly decreased during the high cholesterol diet infusion
in cynomolgus macaques. Rates of appearance of lymph total cholesterol mass
were significantly increased during infusion of high cholesterol diets due
to a statistically significant increase in cholesteryl ester transport
rates. This increase was due in large part to the preferential
esterification of exogenous cholesterol that was incorporated into lymph
chylomicrons and VLDL. The rate of appearance in lymph of exogenous
cholesterol significantly increased during the high cholesterol diet
infusion while that of endogenous cholesterol decreased. This decrease or
compensation in endogenous cholesterol transport occurred during absorption
of increased levels of dietary cholesterol and apparently was due to an
approximate 50% decrease in the absorption of lumenal cholesterol. Our data
demonstrated that there was not a simple one-to-one relationship between
the amount of isotopic dietary cholesterol absorbed from the intestinal
lumen and the mass of cholesterol moved into lymph via the intestine.
Rather, the amount of sterol transported into the body depends on the
degree of the decrease in endogenous cholesterol transport and probably on
the efficiency of cholesterol esterification during absorption.

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Copyright © 1983 by the American Society for Biochemistry and Molecular Biology.
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