HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klein, R. L. Articles by Rudel, L. L. Search for Related Content PubMed PubMed Citation Articles by Klein, R. L. Articles by Rudel, L. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 24, 343-356, Copyright © 1983 by Lipid Research, Inc.

ARTICLES

Cholesterol absorption and transport in thoracic duct lymph lipoproteins of nonhuman primates. Effect of dietary cholesterol level

RL Klein and LL Rudel

The effect of dietary cholesterol level on cholesterol absorption and its subsequent transport in thoracic duct lymph lipoproteins was studied in two species of nonhuman primates, namely the African green monkey (Cercopithecus aethiops) and the cynomolgus macaque (Macaca fascicularis). Each animal served as its own control because each received sequential, intraduodenal infusions of two fat-rich liquid diets that differed only in the amounts of cholesterol. The percentage of dietary cholesterol absorbed was calculated by dividing the rate of appearance of exogenous cholesterol radioactivity in lymph by the rate of infusion of dietary cholesterol radioactivity at a time when lymph cholesterol specific activity was constant relative to that of diet. The percentage of dietary cholesterol absorbed was similar during both diet infusions in African green monkeys but was significantly decreased during the high cholesterol diet infusion in cynomolgus macaques. Rates of appearance of lymph total cholesterol mass were significantly increased during infusion of high cholesterol diets due to a statistically significant increase in cholesteryl ester transport rates. This increase was due in large part to the preferential esterification of exogenous cholesterol that was incorporated into lymph chylomicrons and VLDL. The rate of appearance in lymph of exogenous cholesterol significantly increased during the high cholesterol diet infusion while that of endogenous cholesterol decreased. This decrease or compensation in endogenous cholesterol transport occurred during absorption of increased levels of dietary cholesterol and apparently was due to an approximate 50% decrease in the absorption of lumenal cholesterol. Our data demonstrated that there was not a simple one-to-one relationship between the amount of isotopic dietary cholesterol absorbed from the intestinal lumen and the mass of cholesterol moved into lymph via the intestine. Rather, the amount of sterol transported into the body depends on the degree of the decrease in endogenous cholesterol transport and probably on the efficiency of cholesterol esterification during absorption.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. J. Field, K. Watt, and S. N. Mathur Ezetimibe interferes with cholesterol trafficking from the plasma membrane to the endoplasmic reticulum in CaCo-2 cells J. Lipid Res., August 1, 2007; 48(8): 1735 - 1745. [Abstract] [Full Text] [PDF]

				R. E. Temel, R. G. Lee, K. L. Kelley, M. A. Davis, R. Shah, J. K. Sawyer, M. D. Wilson, and L. L. Rudel Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2 J. Lipid Res., November 1, 2005; 46(11): 2423 - 2431. [Abstract] [Full Text] [PDF]

				K. Liang, C. H. Kim, and N. D. Vaziri HMG-CoA reductase inhibition reverses LCAT and LDL receptor deficiencies and improves HDL in rats with chronic renal failure Am J Physiol Renal Physiol, March 1, 2005; 288(3): F539 - F544. [Abstract] [Full Text] [PDF]

				N. D. Vaziri and K. Liang ACAT inhibition reverses LCAT deficiency and improves plasma HDL in chronic renal failure Am J Physiol Renal Physiol, November 1, 2004; 287(5): F1038 - F1043. [Abstract] [Full Text] [PDF]

				N.D. Vaziri and K.H. Liang Acyl-Coenzyme A:Cholesterol Acyltransferase Inhibition Ameliorates Proteinuria, Hyperlipidemia, Lecithin-Cholesterol Acyltransferase, SRB-1, and Low-Denisty Lipoprotein Receptor Deficiencies in Nephrotic Syndrome Circulation, July 27, 2004; 110(4): 419 - 425. [Abstract] [Full Text] [PDF]

				J. Iqbal, K. Anwar, and M. M. Hussain Multiple, Independently Regulated Pathways of Cholesterol Transport across the Intestinal Epithelial Cells J. Biol. Chem., August 22, 2003; 278(34): 31610 - 31620. [Abstract] [Full Text] [PDF]

				D. Q-H. Wang and M. C. Carey Measurement of intestinal cholesterol absorption by plasma and fecal dual-isotope ratio, mass balance, and lymph fistula methods in the mouse: an analysis of direct versus indirect methodologies J. Lipid Res., May 1, 2003; 44(5): 1042 - 1059. [Abstract] [Full Text] [PDF]

				K. Liang and N. D. Vaziri Upregulation of acyl-CoA:cholesterol acyltransferase in chronic renal failure Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E676 - E681. [Abstract] [Full Text] [PDF]

				D. Q-H. Wang, B. Paigen, and M. C. Carey Genetic factors at the enterocyte level account for variations in intestinal cholesterol absorption efficiency among inbred strains of mice J. Lipid Res., November 1, 2001; 42(11): 1820 - 1830. [Abstract] [Full Text] [PDF]

				R. A. Anderson, C. Joyce, M. Davis, J. W. Reagan, M. Clark, G. S. Shelness, and L. L. Rudel Identification of a Form of Acyl-CoA:Cholesterol Acyltransferase Specific to Liver and Intestine in Nonhuman Primates J. Biol. Chem., October 9, 1998; 273(41): 26747 - 26754. [Abstract] [Full Text] [PDF]