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Journal of Lipid Research, Vol 24, 666-669, Copyright © 1983 by Lipid Research, Inc.

ARTICLES

Retention of gangliosides in serum delipidated by diisopropyl ether-1- butanol extraction

CG Wong and S Ladisch

Extraction with diisopropyl ether-1-butanol is a rapid method for the delipidation of serum without protein denaturation. We sought to confirm that this solvent system would also extract the highly polar acidic glycosphingolipids, gangliosides. In fact, however, both endogenous serum gangliosides and radiolabeled rat brain gangliosides added to serum were nearly quantitatively retained (87.5% and 98.7%, respectively) in the aqueous phase after two extractions. Therefore, while useful for the extraction of most lipids, this delipidation procedure cannot be used to remove gangliosides from serum or plasma.
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This article has been cited by other articles:


Home page
 Annals of Clinical & Laboratory ScienceHome page
X. Zhang and F. L. Kiechle
Glycosphingolipids in Health and Disease
Ann. Clin. Lab. Sci., January 1, 2004; 34(1): 3 - 13.
[Abstract] [Full Text] [PDF]


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