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Journal of Lipid Research, Vol 25, 1132-1139, Copyright © 1984 by Lipid Research, Inc.

ARTICLES

Separation of brain monosialoganglioside molecular species by high- performance liquid chromatography

H Kadowaki, JE Evans and RH McCluer

A method for the separation of molecular species of brain monosialogangliosides by high-performance liquid chromatography is described. GM4, GM3, GM2, and GM1 were purified from human brain and their individual molecular species were separated on a C18 reversed- phase column. Peaks were identified by mass spectrometry of the intact ganglioside, by gas-liquid chromatography of the fatty acids, and by high-performance liquid chromatography of the long chain bases. A characteristic elution sequence of molecular species permitted their identification based upon their retention times on the reversed-phase column.
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