Journal of Lipid Research, Vol 25, 135-147, Copyright © 1984 by Lipid Research, Inc.
Kinetics and thermodynamics of the formation of mixed micelles of egg phosphatidylcholine and bile salts
N Rajagopalan and S Lindenbaum
The kinetics of dissolution of dispersions of egg phosphatidylcholine
(lecithin) by bile salts was studied by observing the decrease in turbidity
as mixed micelles of lecithin and bile salt were formed. The rate of
dissolution of lecithin corresponding to the formation of mixed micelles
was studied in the presence of dihydroxy bile salts, sodium deoxycholate,
sodium chenodeoxycholate, sodium ursodeoxycholate, and one trihydroxy bile
salt, sodium cholate. The rate of dissolution of lecithin and mixed micelle
formation decreased in the order: chenodeoxycholate greater than
deoxycholate greater than cholate greater than ursodeoxycholate. Kinetic
solvent isotope studies in D2O, along with measurement of enthalpies of
mixed micelle formation both in H2O and D2O, appear to indicate that the
formation and stabilization of mixed micelles is related to
"hydrophobicity" as estimated by high performance liquid chromatography
retention factors.
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