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Journal of Lipid Research, Vol 25, 361-368, Copyright © 1984 by Lipid Research, Inc.

ARTICLES

Identification of bile alcohols in urine from healthy humans

M Kuwabara, T Ushiroguchi, K Kihira, T Kuramoto and T Hoshita

Bile alcohols present in urine from healthy adults were studied. Urine was extracted with octadecylsilane-bonded silica gel, and a neutral fraction and a glucuronide fraction were isolated by ion exchange chromatography on piperidinohydroxypropyl Sephadex LH-20. Following hydrolysis of the glucuronide fraction with beta-glucuronidase and purification by silica gel column chromatography, the bile alcohols were analyzed by a combined gas-liquid chromatography-mass spectrometry. By direct comparison with reference standards, the structures of four major bile alcohols were elucidated as follows: 27- nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25- pentol ; 5 beta- cholestane-3 alpha,7 alpha,12 alpha,24, 25- pentol ; 5 beta-cholestane- 3 alpha,7 alpha,12 alpha,25,26- pentol ; and 5 beta-cholestane-3 alpha,7 alpha,12 alpha,26,27- pentol . The daily excretion of these four bile alcohols in urine was 0.5-1.0 mumoles. After purification by silica gel column chromatography, the neutral fraction was analyzed by a combined gas-liquid chromatography-mass spectrometry. The major bile alcohol excreted in urine as the unconjugated form was 27-nor-5 beta- cholestane-3 alpha,7 alpha,12 alpha,24,25- pentol . The daily excretion of the unconjugated C26 pentol (0.02-0.05 mumoles) was far lower than that of the conjugated C26 pentol (0.34-0.86 mumoles).
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				M. Une, S. Takenaka, T. Kuramoto, K. Fujimura, T. Hoshita, and K. Kihira Structural and biosynthetic studies of a principal bile alcohol, 27-nor-5{beta}-cholestane-3{alpha},7{alpha},12{alpha},24,25-pentol, in human urine J. Lipid Res., October 1, 2000; 41(10): 1562 - 1567. [Abstract] [Full Text]