HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lundberg, B. Articles by Suominen, L. Search for Related Content PubMed PubMed Citation Articles by Lundberg, B. Articles by Suominen, L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 25, 550-558, Copyright © 1984 by Lipid Research, Inc.

ARTICLES

Preparation of biologically active analogs of serum low density lipoprotein

B Lundberg and L Suominen

A method for the preparation of stable and water-soluble analogs of low density lipoprotein (LDL) is presented. The experimental protocols start with the preparation of a cholesteryl ester/phospholipid microemulsion by a combined injection-sonication procedure and delipidation of apoprotein B (apoB) with sodium deoxycholate (NaDOC). The association of lipid microemulsion and NaDOC-solubilized apoB is achieved by incubation and sonication of the components above the melting point of the cholesteryl ester. The reconstituted model LDL (m- LDL) proved to be quite homogeneous both with respect to particle size and composition. Negative-stain electron microscopy shows spherical particles with a mean diameter of 21 nm. The mean density of the reconstituted LDL was 1.07 g/ml as determined by sucrose density gradient centrifugation. The reconstituted LDL retained its beta- mobility on agarose gel electrophoresis, and sodium dodecyl sulfate (SDS)-gel electrophoresis showed no degradation of apoB during the reconstitution procedures. Studies of biological activity showed that the m-LDL particles are bound, incorporated, and degraded by human fibroblasts in a way similar to native LDL. The reconstituted m-LDL has potential use for metabolic, physiochemical, and enzymatic studies of lipoproteins.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. S. Kruth, W.-Y. Zhang, S. I. Skarlatos, and F.-F. Chao Apolipoprotein B Stimulates Formation of Monocyte-Macrophage Surface-connected Compartments and Mediates Uptake of Low Density Lipoprotein-derived Liposomes into these Compartments J. Biol. Chem., March 12, 1999; 274(11): 7495 - 7500. [Abstract] [Full Text] [PDF]

				P. C. N. Rensen, R. M. Schiffelers, A. J. Versluis, M. K. Bijsterbosch, M. E. M. J. Van Kuijk-Meuwissen, and T. J. C. Van Berkel Human Recombinant Apolipoprotein E-Enriched Liposomes Can Mimic Low-Density Lipoproteins as Carriers for the Site-Specific Delivery of Antitumor Agents Mol. Pharmacol., September 1, 1997; 52(3): 445 - 455. [Abstract] [Full Text]