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Journal of Lipid Research, Vol 25, 651-652, Copyright © 1984 by Lipid Research, Inc.

ARTICLES

Improved version of the Kean partition assay for cerebroside sulfate

NS Radin

In the Kean method for the colorimetric determination of sulfatide (1968. J. Lipid Res. 9: 319-327), the lipid is partitioned together with a blue cationic compound between two phases formed from chloroform, methanol, and water. The blue cation enters the chloroform- rich phase only as an ion pair with the lipid. This method has been improved by the use of a new mixture of solvents in which the desired layer floats above the excess dye. The lower volatility of the new solvent system improves the reproducibility of the technique.
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