HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jungalwala, F. B. Articles by McCluer, R. H. Search for Related Content PubMed PubMed Citation Articles by Jungalwala, F. B. Articles by McCluer, R. H. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 25, 738-749, Copyright © 1984 by Lipid Research, Inc.

ARTICLES

Compositional and molecular species analysis of phospholipids by high performance liquid chromatography coupled with chemical ionization mass spectrometry

FB Jungalwala, JE Evans and RH McCluer

High performance liquid chromatography (HPLC) was combined with chemical ionization mass spectrometry (CIMS) by the use of a moving- belt interface. The technique was employed for the analysis of naturally occurring phospholipids. Positive and negative ion mass spectra of various phospholipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, and sphingomyelin were obtained in the chemical ionization mode with ammonia or methane as the reagent gas. Specific ions for individual phospholipid "bases" were identified. These ions were used in specific ion monitoring of the phospholipids during HPLC- CIMS. CIMS of each phospholipid also provided extensive information on the molecular species of the individual class of phospholipids. Relative abundance of different molecular species of each phospholipid as determined by CIMS agreed well with the results obtained by gas- liquid chromatography. Rat brain phospholipids were analyzed by HPLC- CIMS in about 15 minutes. Routinely, about 5 micrograms of individual phospholipid was analyzed by HPLC-CIMS, however, with specific ion monitoring the method provides a detection capability at the subnanogram level.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Houjou, J. Hayakawa, R. Watanabe, Y. Tashima, Y. Maeda, T. Kinoshita, and R. Taguchi Changes in molecular species profiles of glycosylphosphatidylinositol anchor precursors in early stages of biosynthesis J. Lipid Res., July 1, 2007; 48(7): 1599 - 1606. [Abstract] [Full Text] [PDF]

				K. L. Krenzer, M. Reza Dana, M. D. Ullman, J. M. Cermak, D. B. Tolls, J. E. Evans, and D. A. Sullivan Effect of Androgen Deficiency on the Human Meibomian Gland and Ocular Surface J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4874 - 4882. [Abstract] [Full Text]

				J. F. H. M. Brouwers, E. A. A. M. Vernooij, A. G. M. Tielens, and L. M. G. van Golde Rapid separation and identification of phosphatidylethanolamine molecular species J. Lipid Res., January 1, 1999; 40(1): 164 - 169. [Abstract] [Full Text]

				B. Brugger, G. Erben, R. Sandhoff, F. T. Wieland, and W. D. Lehmann Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry PNAS, March 18, 1997; 94(6): 2339 - 2344. [Abstract] [Full Text] [PDF]