Journal of Lipid Research, Vol 25, 770-779, Copyright © 1984 by Lipid Research, Inc.
In vitro effect of Triton WR-1339 on canine plasma high density lipoproteins
K Yamamoto, R Byrne, C Edelstein, B Shen and AM Scanu
We studied the effect in vitro of various concentrations of Triton WR- 1339
on normolipidemic canine plasma and on the high density lipoproteins (HDL)
isolated from this plasma by ultracentrifugation. As a preamble to this
study, we established that Triton WR-1339 has a unimer molecular weight of
4,500, a micellar molecular weight of 180,000, and a critical micellar
concentration (CMC) of 0.018 mM or 0.008 g/dl. Above its CMC, Triton
WR-1339 in concentrations between 2 and 10 mg/ml induced
concentration-dependent structural changes in HDL which were characterized
by a progressive displacement of apoA-I from the HDL surface without loss
of lipids. The addition of Triton WR-1339 to the HDL particles modified
their electrophoresis mobility and caused an increase in size (95 +/- 5 A
to 114 +/- 7 A). At the extreme Triton WR-1339 concentrations utilized in
these studies (10 mg/ml) disruption of the HDL particles occurred; at this
stage, the original, relatively homogeneous, spherical HDL particles were
replaced by a heterogeneous population ranging in size between 50 and 250
A, representing complexes of Triton WR-1339 with lipids essentially free of
apoA-I which could be sedimented by ultracentrifugation. The effects of
Triton WR-1339 on whole plasma or isolated HDL were comparable. These
studies indicate that Triton WR-1339 in vitro alters HDL in a
concentration-dependent manner and that these changes vary from a
displacement of apoA-I from the HDL surface to a state where all lipids are
solubilized into the Triton WR-1339 micellar phase and are driven away from
the protein moiety.(ABSTRACT TRUNCATED AT 250 WORDS)
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